Projets de recherche

Research Projects



Research Projects of LBC Laboratory

Authors:

Year of completion of the grant:

Research at the LBC is focused on droplet-based microfluidics for applications in biology. In recent years we have developed this powerful technology that we now aim to use and further develop to address challenges in the following fields:
- Evolutionary biology
- Drug Discovery
- Industrial biochemistry, bioprocesses
- Diagnostics
- Therapeutics

Our research is balanced between fundamental and applied projects, and you will find examples of both categories below.
- Our core technology: droplet-based microfluidics
- Fundamental research: Origin of Life / RNA world
- Digital Diagnostics
- Drug Discovery: Antibodies/Hybridoma/B-cell screening
- Drug Discovery: Chemical Library Screening
- Industrial Biotechnology: Screening for Enzymatic Activities in Microorganisms

Détail Research Projects of LBC Laboratory


Research project of Systems Biology of Cell Polarity and Cell Division lab.

Authors:

Year of completion of the grant:

Our team studies cell polarization, a process that involves a reorganization of the cell cytoskeleton and movement of cellular organelles, usually triggered by external cues. We are particularly interested in cell polarity in the context of cell migration and cell division.
We develop and use innovative tools based on nano and micro-fabrication techniques, to control and modulate the main physical and chemical parameters of the cell microenvironment. These tools are coupled with high quality quantitative microscopy, and used alongside molecular and cell biology techniques, to obtain a quantitative description of the cell behavior. As well as highlighting new basic concepts about cell polarity, our multidisciplinary approach leads to the development of novel tools with potential applications in biomedical research.

Our main fundings are:

- ERC consolidator grant (coordination by M. Piel), in 2012 for project PROMICO: cell proliferation under external constrains (1,5 M€)
- Labex IPGG (2012) (coordination by M. Piel), immune system on-a-chip (150 k€)
- INCA PLBIO (2011) (coordination by M. Piel), “Mitotic cell rounding mechanisms and function” project (450 k€). Consortium: M.Piel, B. Baum (UCL, UK), JF Joanny (I.C.), G. Capello (I.C.)
- Ligue contre le Cancer (2010 fixed subvention) (coordination by M. Piel), “Mitotic cell rounding mechanisms and function” project (50 k€)
- ANR Piribio (2009) (coordinated with M. Piel), MICEMICO project (Cell migration in confined environment) (600 k€). Consortium M. Piel, AM Lennon Duménil (I.C.), V. Soumelis (I.C.), R. Voituriez (LPTMC, UPMC), D. Vignjevic (I.C.)
- InNaBioSanté (2009) (coordination by AM Lennon Duménil), MICEMICO project (400 k€). Consortium M. Piel, AM Lennon Duménil (I.C.), V. Soumelis (I.C.), R. Voituriez (LPTMC, UPMC), D. Vignjevic (I.C.)

Détail Research project of Systems Biology of Cell Polarity and Cell Division lab.

Détail Research project of Systems Biology of Cell Polarity and Cell Division lab.

Research Projects of Colloids and Divided Materials Laboratory

Authors:

Year of completion of the grant:

- Material Science
- Physics of Colloids and and Interfaces
- Physicochemistry for Microbiology
- New technologies & Devices

Détail Research Projects of Colloids and Divided Materials Laboratory


Research Projects of ABCD LOCCO Laboratory

Authors:

Year of completion of the grant:

Our research is currently focused around the following projects :

New tools for super-resolution imaging : we aim to implement novel methods for single molecule detection, with a special emphasis to 3D imaging in live cells.

Target search of DNA Binding proteins : We want to understand the mechanisms by which DNA-binding proteins find their target site in live eukaryotic cells.

Magnetic manipulation of intracellular signaling : our goal is to use magnetic beads to control and manipulate signalling activity inside living cells.

Optogenetic control of cell polarity : we want to use light-based tools to control the localization and activity of molecules, in order to analyze at a systems level the processes by which cells polarize during cell migration and division.

Détail Research Projects of ABCD LOCCO Laboratory

Détail Research Projects of ABCD LOCCO Laboratory

Projects of Macromolecules et Microsystems in Biology and Medecine lab.

Authors:

Year of completion of the grant:

The philosophy of the team is to use physics, chemistry and soft matter science as a "toolbox" to develop new tools and concepts for biology and medicine. It has focused during the last years on microfluidics, combining fundamental studies, the invention and development of new technological concepts, and application to problems in the real life. It has, in particular, developed expertise in new materials and new fabrication strategies for soft lithography, in the use of self-assembly in lab-on-chips, and in droplet microfluidics. Applications developed by the team involve e.g. the selection and study of circulating tumour cells as a support to cancer diagnosis, the search of biomarkers of Alzheimer's disease, low-cost DNA platform for diagnosis and environmental genetic analysis, or cell culture in microfluidic format.

Our group has three main lines of research :
The first is the development of bioanalytical tools and methods. Our group is a pioneer in microfluidics and lab on chips, developing in the area innovative technologies: magnetic and convective self-assembly, flow control, non-conventional microfabrication strategies and surface treatments, high throughput droplet microfluidics. Using these technologies, our group is developing several diagnosis-oriented projects in collaboration with clinicians, e.g.:

• Development of new media and strategies for mutation analysis (now in use in routine in several hospitals in France).

• Capture and molecular typing of tumour cells from patients, for the evaluation of metastatic relapse and treatment orientation. We are the coordinator of the European Project Caminems (cf charte d'aide à la lecture : un acronyme qui se lit comme un mot, sans l'épeler, s'écrit en minuscules) on this topic, and collaborating with several hospitals and research groups in France and abroad.

• Early diagnosis of neurodegenerative diseases (prion diseases, Alzheimer) by microfluidic methods, within the European consortium NeuroTAS

• Original systems for the oriented culture of neurones, and the study of neurons degenerescence

• Portable “point of care” microfluidic device for fast genetic analysis of pathogens, and the diagnosis of nosocomial infections (ANR project “Redloc”)

Détail Projects of Macromolecules et Microsystems in Biology and Medecine lab.

Détail Projects of Macromolecules et Microsystems in Biology and Medecine lab.

Research project of MMN laboratory

Authors:

Year of completion of the grant:

Since its creation, the lab has explored many subjects related to physics, chemistry and biology. Its current key topics are the conception of new actuators that could compete with PDMS valves, which stressed the link between microfludics and microelectronics (in collaboration with PPMD). Another topic is the in vivo targeted delivery of molecules using micrometric droplets and ultrasound. We also work on nano-velocimetry, which allows to study the flows at 100 nm of a wall, the study of complex fluids in confined spaces, oil flows enhanced by polymer solutions in microfluidic networks (the most realistic ever made, in terms of size and wettability), drops steering with thermics, production of platelets in microsystems, microfluidic assembly of colloids or the analysis at the single cell scale of its expression, in high throughput conditions.
The group consists of about twenty researchers, engineers and students. While developing its own research topics, it lies at the heart of a particularly rich, collaborative network.

Détail Research project of MMN laboratory

Détail Research project of MMN laboratory

Research project of NBP laboratory

Authors:

Year of completion of the grant:

In the past decade, local force measurement techniques such as atomic force microscopy and optical tweezers were used to study elastic properties and mechanically induced structural transitions of nucleic acids at the single molecule level.



Research project of Biology inspired physics at meso-scales lab.

Authors:

Year of completion of the grant:

E. coli is not only chemotactic, i.e. this bacterium swims towards a source of attractants (food, oxygen…), it also expresses some of these attractants.


Détail Research project of Biology inspired physics at meso-scales lab.

Research Projects of ABCD Biophysics Lab

Authors:

Year of completion of the grant:

The current active research projects of the lab are summarised below:


1. Single molecule micromanipulations are devoted to the study of DNA and DNA-protein interactions based on a magnetic tweezers system. The lab is currently working at:
– an in-vitro reconstitution of the replication fork
– a new sequencing method of single DNA molecules
– the action of diverse helicases, topoisomerases and translocases on DNA

2. At the cell level, the lab is interested in how in-vivo replication forks can restart after controlled arrest. This project involves single molecule fluorescence and microfluidics.

3. The part of the lab dedicated to developmental biology uses a 2-photon set-up to influence cell lineage in the course of embryo development by using photo-activable molecules which can be rapidly triggered in a single cell at a given time.

4. At the population level, the lab is focusing on the role of phenotypic variability for iron regulation in Pseudomonas aeruginosa population. The technics developed for this purpose are essentially: microscopy, image analysis, microfluidics and laser ablation. We are also trying to implement a long term evolution experiment of Escherichia coli in a temperature gradient.

Détail Research Projects of ABCD Biophysics Lab


Research Projects of Pôle Microfluidique

Authors:

Year of completion of the grant:

- Synthetic cellular microenvironments
- Bioengineering of Liquid Interfaces
- Synthetic epigenetics
- Light-driven microfluidics for chemistry and biology


Détail Research Projects of Pôle Microfluidique

15 projects.
<Previous - 2 >