Université PSL

A new ANR grant for Sophie Griveau and coworkers

A new ANR grant for Sophie Griveau and coworkers
- Institute of Chemistry for Life and Health Sciences, iCLeHS, UMR 8060, Team SEISAD
(Sophie GRIVEAU)
- Laboratory Biologie de la Reproduction, Environnement, Epigénétique & Développement - Team EPEE - INRAe UMR 1198 (Véronique DURANTHON)
- Laboratory P.A.S.T.E.U.R – UMR – 8640 - Team NanoBiosciences and MicroSystems
(NBMS) (Laurent THOUIN)

In western developed countries, about 3% of births proceed from assisted reproductive technologies, most of them involving in vitro culture of the embryos. It was recently shown that oxidative stress, experienced by the embryo during the culture period greatly affects the success of such embryo biotechnologies and may impact the future health of the individuals born from these biotechnologies.
In this context, the project EmbryoElecSense will develop innovative multiparametric microdevices to monitor in real time, in situ and label-free manner, the oxidative stress during in vitro embryo development during the pre-implantation period (4 days), at the level of embryo population and then of one single embryo. Specific devices will be conceived, with the incorporation of several microelectrodes in the bottom of culture wells, for the simultaneous electrochemical monitoring of ROS and RNS. Such non-invasive approach will bring high
selectivity, high temporal and spatial resolutions. The monitoring will be performed in situ during the preimplantation period of embryo development within an incubator.
This project will allow establishing any correlations between intra and extracellular production of ROS and RNS during embryo development. Such analysis will allow an in depth analysis of the kinetics of intra/extra oxidative stress. In addition it will also provide quantitative information’s of ROS and RNS levels during the different stages of embryo development. Finally, this should allow to examine correlations between intra and extra production of reactive species, indicative of the redox status of embryos. The proposed technologies should thus provide a tool for non-invasive monitoring of embryo development during in vitro culture.